Leica cryoCLEM Stellaris 8 confocal microscope
Description: This upright confocal microscope from Leica is equipped with a cryo-stage for imaging vitrified specimens on electron microscopy grids under constant cooling with liquid nitrogen. Available fluorescence excitation light sources include a 405 nm pulsed laser, a white light pulsed laser, and 488 nm, 561 nm, and 633 nm cw lasers. The microscope is equipped with four hybrid detectors and has a FLIM unit. In addition, the microscope can be operated in widefield mode and has LED a fluorescence widefield excitation light source. The microscope is programmable via the Navigator module of the Leica LASX software. The microscope is operated in a biological safety level 2 (BSL2) laboratory.
Main applications: Light cryo-microscopy, confocal microscopy, FLIM, FRET, widefield microscopy, correlative light- and electron-cryo-microscopy.