Publications
2023
Sterrenberg VT, Stalling D, Knaack JIH, Soh TK, Bosse JB, Meier C (2023) A TriPPPro-Nucleotide Reporter with Optimized Cell-Permeable Dyes for Metabolic Labeling of Cellular and Viral DNA in Living Cells. Angew Chem Int Ed Engl. 62(38):e202308271. doi: 10.1002/anie.202308271.
Bogdanow B, Gruska I, Mühlberg L, Protze J, Hohensee S, Vetter B, Bosse JB, Lehmann M, Sadeghi M, Wiebusch L, Liu F (2023) Spatially resolved protein map of intact human cytomegalovirus virions. Nat Microbiol. doi: 10.1038/s41564-023-01433-8.
Lewis HC, Kelnhofer-Millevolte LE, Brinkley MR, Arbach HE, Arnold EA, Sanders S, Bosse JB, Ramachandran S, Avgousti DC (2023) HSV-1 exploits host heterochromatin for nuclear egress. J Cell Biol. 222(9):e202304106. doi: 10.1083/jcb.202304106.
Sanders S, Jensen Y, Reimer R, Bosse JB (2023) From the beginnings to multidimensional light and electron microscopy of virus morphogenesis. Adv Virus Res. 116:45-88. doi: 10.1016/bs.aivir.2023.05.001.
Soh TK, Pfefferle S, Wurr S, von Possel R, Oestereich L, Rieger T, Uetrecht C, Rosenthal M, Bosse JB (2023) A validated protocol to UV-inactivate SARS-CoV-2 and herpesvirus-infected cells. PLoS One. 18(5):e0274065. doi: 10.1371/journal.pone.0274065
Bosse JB, Brune W (2023) Viral dew: Phase separation and the formation of viral replication compartments. PLoS Pathog. 19(2):e1011145. doi: 10.1371/journal.ppat.1011145
2022
Borst EM, Harmening S, Sanders S, Caragliano E, Wagner K, Lenac Roviš T, Jonjić S, Bosse JB, Messerle M (2022) A Unique Role of the Human Cytomegalovirus Small Capsid Protein in Capsid Assembly. mBio. e0100722. doi: 10.1128/mbio.01007-22
Flomm FJ, Soh TK, Schneider C, Wedemann L, Britt HM, Thalassinos K, Pfitzner S, Reimer R, Grünewald K, Bosse JB (2022) Intermittent bulk release of human cytomegalovirus. PLoS Pathog. 18(8):e1010575. doi: 10.1371/journal.ppat.1010575.
Caragliano E, Brune W, Bosse JB (2022) Herpesvirus Replication Compartments: Dynamic Biomolecular Condensates? Viruses. 14(5):960. doi: 10.3390/v14050960.
Wedemann L, Flomm FJ,
Bosse JB (2022) The unconventional way out - Egress of HCMV through multiviral bodies. Mol Microbiol.
doi: 10.1111/mmi.14946
Caragliano E, Bonazza S, Frascaroli G, Tang J, Soh TK, Grünewald K,
Bosse JB, Brune W (2022) Human cytomegalovirus forms phase-separated compartments at viral genomes to facilitate viral replication. Cell Reports. 38(10):110469.
doi: 10.1016/j.celrep.2022.110469.
2021
Aho V, Salminen S, Mattola S, Gupta A, Flomm F, Sodeik B, Bosse JB, Vihinen-Ranta M (2021) Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane. PLoS Pathog. 17(12):e1010132. doi: 10.1371/journal.ppat.1010132
Pfitzner S, Bosse JB, Hofmann-Sieber H, Flomm F, Reimer R, Dobner T, Grünewald K, Franken LE (2021). Human Adenovirus Type 5 Infection Leads to Nuclear Envelope Destabilization and Membrane Permeability Independently of Adenovirus Death Protein. Int J Mol Sci. 22(23):13034.
Jung JM, Ching W, Baumdick ME, Hofmann-Sieber H, Bosse JB, Koyro T, Möller KJ, Wegner L, Niehrs A, Russu K, Ohms M, Zhang W, Ehrhardt A, Duisters K, Spierings E, Hölzemer A, Körner C, Jansen SA, Peine S, Königs I, Lütgehetmann M, Perez D, Reinshagen K, Lindemans CA, Altfeld M, Belderbos M, Dobner T, Bunders MJ (2021) KIR3DS1 directs NK cell-mediated protection against human adenovirus infections. Sci Immunol;6(63):eabe2942. doi: 10.1126/sciimmunol.abe2942.
Rink MR, Baptista MAP, Flomm FJ, Hennig T, Whisnant AW, Wolf N, Seibel J, Dölken L, Bosse JB (2021) Concatemeric Broccoli reduces mRNA stability and induces aggregates. PLoS One. 16(8):e0244166. doi: 10.1371/journal.pone.0244166.
Pearson VR, Bosse JB, Koyuncu OO, Scherer J, Toruno C, Robinson R, Abegglen LM, Schiffman JD, Enquist LW, Rall GF (2021) Identification of African Elephant Polyomavirus in wild elephants and the creation of a vector expressing its viral tumor antigens to transform elephant primary cells. PLoS One;16(2): e0244334. doi: 10.1371/journal.pone.0244334.
2020
Soh TK, Bosse JB (2020) What is in a Viral Stock: Perspectives and Current Limitations of Flow Virometry. Preprints, 2020110409. doi: 10.20944/preprints202011.0409.v1
Flomm FJ, Soh TK, Schneider C, Britt HM, Thalassinos K, Pfitzner S, Reimer R, Grünewald K, Bosse JB (2020) Intermittent bulk release of human cytomegalovirus through multivesicular bodies. bioRxiv.424954. doi: https://doi.org/10.1101/2020.12.31.424954
Rink MR, Baptista MAP, Hennig T, Whisnant AW, Wolf N, Seibel J, Dölken L, Bosse JB (2020) Concatemeric Broccoli reduces mRNA stability, aggregates and induces p-body formation. bioRxiv. 414458. doi: https://doi.org/10.1101/2020.12.07.414458
Pfitzner S, Hofmann-Sieber H, Bosse JB, Franken LE, Grünewald K, Dobner T (2020) Fluorescent protein tagging of adenoviral proteins pV and pIX reveals 'late virion accumulation compartment'. PLoS Pathog.16(6): e1008588. doi: 10.1371/journal.ppat.1008588.
2019
Wang C, Lunelli M, Zschieschang E, Bosse JB, Thuenauer R, Kolbe M (2019) Role of flagellar hydrogen bonding in Salmonella motility and flagellar polymorphic transition. Molecular Microbiology 112:1519–1530.
Wyler E, Franke V, Menegatti J, Kocks C, Boltengagen A, Praktiknjo S, Walch-Rückheim B, Bosse JB, Rajewsky N, Grässer F, Akalin A , Landthaler M (2019) Single-cell RNA-sequencing of herpes simplex virus 1-infected cells connects NRF2 activation to an antiviral program. Nat Commun 10:1–14.
Langeneckert AE, Lunemann S, Martrus G, Salzberger W, Hess LU, Ziegler AE, Poch T, Ravichandran G, Matschl U, Bosse JB, Tiegs G, Fischer L, Koch M, Herkel J, Oldhafer KJ, Schramm C, Altfeld M (2019) CCL21‐expression and accumulation of CCR7+ NK cells in livers of patients with primary sclerosing cholangitis. Eur J Immunol 49:758–769.
Flomm F, Borst EM, Günther T, Reimer R, de Vries L, Schneider C, Grundhoff A, Grünewald K, Messerle M, Bosse JB (2019) Human cytomegalovirus nuclear capsid motility is non-directed and independent of nuclear actin bundles. bioRxiv 641266.
2018
Nauth T, Huschka F, Schweizer M, Bosse JB, Diepold A, Failla AV, Steffen A, Stradal T, Wolters M, Aepfelbacher M (2018) Visualization of translocons in Yersinia type III protein secretion machines during host cell infection. PLoS Pathog 14:e1007527.
2017
Buch A, Müller O, Ivanova L, Döhner K, Bialy D, Bosse JB, Pohlmann A, Binz A, Hegemann M, Nagel C (2017) Inner tegument proteins of Herpes Simplex Virus are sufficient for intracellular capsid motility in neurons but not for axonal targeting. PLoS Pathog 13:e1006813.
Flomm F, Bosse JB (2017) Potential mechanisms facilitating herpesvirus-induced nuclear remodeling: how are herpesvirus capsids able to leave the nucleus? Future Virology 12:583–592.
2016
Bosse JB, Enquist LW (2016) The diffusive way out: herpesviruses remodel the host nucleus, enabling capsids to access the inner nuclear membrane. nucleus 7:13–19.
2015
Bosse JB, Hogue IB, Feric M, Thiberge SY, Sodeik B, Brangwynne CP, Enquist LW (2015) Remodeling nuclear architecture allows efficient transport of herpesvirus capsids by diffusion. Proc Natl Acad Sci USA 112:E5725–E5733.
Bosse JB, Tanneti NS, Hogue IB, Enquist LW (2015) Open led illuminator: A simple and inexpensive LED illuminator for fast multicolor particle tracking in neurons. PLoS ONE 10:e0143547.
Hogue IB, Bosse JB, Engel EA, Scherer J, Hu J, del Rio T, Enquist LW (2015) Fluorescent protein approaches in alpha herpesvirus research. Viruses 7:5933–596
Hagen C, Dent KC, Zeev-Ben-Mordehai T, Grange M, Bosse JB, Whittle C, Klupp BG, Siebert CA, Vasishtan D, Bäuerlein FJB, Cheleski J, Werner S, Guttmann P, Rehbein S, Henzler K, Demmerle J, Adler B, Koszinowski U, Schermelleh L, Schneider G, Enquist LW, Plitzko JP, Mettenleiter TC, Grünewald K (2015) Structural Basis of Vesicle Formation at the Inner Nuclear Membrane. Cell 163: 1692–1701.
2014
Bosse JB, Virding S, Thiberge SY, Scherer J, Wodrich H, Ruzsics Z, Koszinowski UH, Enquist LW, Racaniello VR (2014) Nuclear Herpesvirus Capsid Motility Is Not Dependent on F-Actin. mBio 5:429–14. doi: 10.1128/mBio.01909-14
Pogoda M, Bosse JB, Conzelmann K-K, Koszinowski UH, Ruzsics Z (2014) A modified screening system for loss-of-function and dominant negative alleles of essential MCMV genes. PLoS ONE 9:e94918.
Hogue IB, Bosse JB, Hu J-R, Thiberge SY, Enquist LW (2014) Cellular mechanisms of alpha herpesvirus egress: live cell fluorescence microscopy of pseudorabies virus exocytosis. PLoS Pathog 10:e1004535. doi: 10.1371/journal.ppat.1004535
2013
Ruzsics Z, Borst EM, Bosse JB, Brune W, Messerle M (2013) Manipulating cytomegalovirus genomes by BAC mutagenesis: strategies and applications, p 37–57.
Wagner FM, Brizic I, Prager A, Trsan T, Arapovic M, Lemmermann NA, Podlech J, Reddehase MJ, Lemnitzer F, Bosse JB, Gimpfl M, Marcinowski L, MacDonald M, Adler H, Koszinowski UH, Adler B (2013) The Viral Chemokine MCK-2 of Murine Cytomegalovirus Promotes Infection as Part of a gH/gL/MCK-2 Complex. PLoS Pathog 9:e1003493. doi: 10.1371/journal.ppat.1003493
Granstedt AE, Bosse JB, Thiberge SY, Enquist LW (2013) In vivo imaging of alphaherpesvirus infection reveals synchronized activity dependent on axonal sorting of viral proteins. Proc Natl Acad Sci USA 110:E3516–E3525.
Bosse JB, Ragues J, Wodrich H (2013) Fast Generation of Stable Cell Lines Expressing Fluorescent Marker Molecules to Study Pathogen Induced Processes. In: Bailer SM, Lieber D (eds) Virus-Host Interactions. Humana Press, Totowa, NJ, pp 153–169
2012
Marcinowski L, Lidschreiber M, Windhager L, Rieder M, Bosse JB, Rädle B, Bonfert T, Györy I, de Graaf M, Prazeres da Costa O, Rosenstiel P, Friedel CC, Zimmer R, Ruzsics Z, Dölken L (2012) Real-time transcriptional profiling of cellular and viral gene expression during lytic cytomegalovirus infection. PLoS Pathog 8:e1002908.
Pogoda M, Bosse JB, Wagner FM, Schauflinger M, Walther P, Koszinowski UH, Ruzsics Z (2012) Characterization of conserved region 2-deficient mutants of the cytomegalovirus egress protein pM53. J Virol 86:12512–12524.
Bosse JB, Bauerfeind R, Popilka L, Marcinowski L, Taeglich M, Jung C, Striebinger H, von Einem J, Gaul U, Walther P, Koszinowski UH, Ruzsics Z (2012) A beta-herpesvirus with fluorescent capsids to study transport in living cells. PLoS ONE 7:e40585.
2011
Maninger S, Bosse JB, Lemnitzer F, Pogoda M, Mohr CA, von Einem J, Walther P, Koszinowski UH, Ruzsics Z (2011) M94 is essential for the secondary envelopment of murine cytomegalovirus. J Virol 85:9254–9267.
2009
Lösing J-B, Di Fiore S, Ritter K, Fischer R, Kleines M (2009) Epstein–Barr virus BDLF2–BMRF2 complex affects cellular morphology. J Gen Virol 90:1440–1449.
2008
Gaspar M, Gill MB, Lösing J-B, May JS, and Stevenson PG (2008) Multiple functions for ORF75c in murid herpesvirus-4 infection. PLoS ONE 3:e2781.
