CSSB Centre for Structural Systems Biology
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Bosse Group

Quantitative Virology

Prof. Dr. Jens Bosse

Group Leader

+49 040 8998 87645
jens.bosse@cssb-hamburg.de

Home Institute

Hannover Medical School
www.mhh.de/

We focus on illuminating the rules that govern the dynamic assembly of viruses in their native cellular environment. Using cutting edge imaging and analysis tools, we can define where, when and how viruses assemble at single-particle precision. Using this knowledge, we aim to identify potential bottlenecks in viral productivity as specific pharmacological target structures.

Research Projects

PREVIOUS AND CURRENT RESEARCH

Viruses assemble in a complex order of consecutive morphogenesis events. Our research aims to illuminate the spatiotemporal regulation of these morphogenesis at the single-particle level. We use three-dimensional live-cell microscopy to quantify the kinetics and dynamics of viral macromolecular complexes in living cells at the single particle level and create computational pipelines to analyze large image datasets. Correlative electron microscopy is used to complement our measurements with ultrastructural data on the cellular organization.

Our research ideally complements projects at CSSB by adding knowledge on the kinetics and dynamics of viral processes to structural approaches.

Currently, we are working on the question of how virus capsid-membrane association and dissociation is regulated. To this end, we cooperate closely with our international Wellcome Trust Collaborators by integrating kinetic models into multimodal frameworks of virus morphogenesis.

Research Team

5

Group Leader

Jens Bosse
Phone:+49 040 8998 87645

E-Mail

Felix Flomm
Phone:+49 40 8998 87710

E-Mail

Saskia Sanders
Phone:+49 40 8998 87646

E-Mail

Jonas Schröder
Phone:+49 40 8998 87647

E-Mail

Dr. Timothy Soh
Phone:+49 40 8998 87711

E-Mail

Publications

2020

Pfitzner S, Hofmann-Sieber H, Bosse JB, Franken LE, Grünewald K, Dobner T (2020) Fluorescent protein tagging of adenoviral proteins pV and pIX reveals 'late virion accumulation compartment'. PLoS Pathog.;16(6):e1008588. doi: 10.1371/journal.ppat.1008588.

2019

Wang C, Lunelli M, Zschieschang E, Bosse JB, Thuenauer R, Kolbe M (2019) Role of flagellar hydrogen bonding in Salmonella motility and flagellar polymorphic transition. Molecular Microbiology 112:1519–1530.

Wyler E, Franke V, Menegatti J, Kocks C, Boltengagen A, Praktiknjo S, Walch-Rückheim B, Bosse JB, Rajewsky N, Grässer F, Akalin A , Landthaler M (2019) Single-cell RNA-sequencing of herpes simplex virus 1-infected cells connects NRF2 activation to an antiviral program. Nat Commun 10:1–14.

Langeneckert AE, Lunemann S, Martrus G, Salzberger W, Hess LU, Ziegler AE, Poch T, Ravichandran G, Matschl U, Bosse JB, Tiegs G, Fischer L, Koch M, Herkel J, Oldhafer KJ, Schramm C, Altfeld M (2019) CCL21‐expression and accumulation of CCR7+ NK cells in livers of patients with primary sclerosing cholangitis. Eur J Immunol 49:758–769.

Flomm F, Borst EM, Günther T, Reimer R, de Vries L, Schneider C, Grundhoff A, Grünewald K, Messerle M, Bosse JB (2019) Human cytomegalovirus nuclear capsid motility is non-directed and independent of nuclear actin bundles. bioRxiv 641266.

2018

Nauth T, Huschka F, Schweizer M, Bosse JB, Diepold A, Failla AV, Steffen A, Stradal T, Wolters M, Aepfelbacher M (2018) Visualization of translocons in Yersinia type III protein secretion machines during host cell infection. PLoS Pathog 14:e1007527.

2017

Buch A, Müller O, Ivanova L, Döhner K, Bialy D, Bosse JB, Pohlmann A, Binz A, Hegemann M, Nagel C (2017) Inner tegument proteins of Herpes Simplex Virus are sufficient for intracellular capsid motility in neurons but not for axonal targeting. PLoS Pathog 13:e1006813.

Flomm F, Bosse JB (2017) Potential mechanisms facilitating herpesvirus-induced nuclear remodeling: how are herpesvirus capsids able to leave the nucleus? Future Virology 12:583–592.

2016

Bosse JB, Enquist LW (2016) The diffusive way out: herpesviruses remodel the host nucleus, enabling capsids to access the inner nuclear membrane. nucleus 7:13–19.

2015

Bosse JB, Hogue IB, Feric M, Thiberge SY, Sodeik B, Brangwynne CP, Enquist LW (2015) Remodeling nuclear architecture allows efficient transport of herpesvirus capsids by diffusion. Proc Natl Acad Sci USA 112:E5725–E5733.

Bosse JB, Tanneti NS, Hogue IB, Enquist LW (2015) Open led illuminator: A simple and inexpensive LED illuminator for fast multicolor particle tracking in neurons. PLoS ONE 10:e0143547.

Hogue IB, Bosse JB, Engel EA, Scherer J, Hu J, del Rio T, Enquist LW (2015) Fluorescent protein approaches in alpha herpesvirus research. Viruses 7:5933–596

Hagen C, Dent KC, Zeev-Ben-Mordehai T, Grange M, Bosse JB, Whittle C, Klupp BG, Siebert CA, Vasishtan D, Bäuerlein FJB, Cheleski J, Werner S, Guttmann P, Rehbein S, Henzler K, Demmerle J, Adler B, Koszinowski U, Schermelleh L, Schneider G, Enquist LW, Plitzko JP, Mettenleiter TC, Grünewald K (2015) Structural Basis of Vesicle Formation at the Inner Nuclear Membrane. Cell 163: 1692–1701.

2014

Bosse JB, Virding S, Thiberge SY, Scherer J, Wodrich H, Ruzsics Z, Koszinowski UH, Enquist LW, Racaniello VR (2014) Nuclear Herpesvirus Capsid Motility Is Not Dependent on F-Actin. mBio 5:429–14. doi: 10.1128/mBio.01909-14

Pogoda M, Bosse JB, Conzelmann K-K, Koszinowski UH, Ruzsics Z (2014) A modified screening system for loss-of-function and dominant negative alleles of essential MCMV genes. PLoS ONE 9:e94918.

Hogue IB, Bosse JB, Hu J-R, Thiberge SY, Enquist LW (2014) Cellular mechanisms of alpha herpesvirus egress: live cell fluorescence microscopy of pseudorabies virus exocytosis. PLoS Pathog 10:e1004535. doi: 10.1371/journal.ppat.1004535

2013

Ruzsics Z, Borst EM, Bosse JB, Brune W, Messerle M (2013) Manipulating cytomegalovirus genomes by BAC mutagenesis: strategies and applications, p 37–57.

Wagner FM, Brizic I, Prager A, Trsan T, Arapovic M, Lemmermann NA, Podlech J, Reddehase MJ, Lemnitzer F, Bosse JB, Gimpfl M, Marcinowski L, MacDonald M, Adler H, Koszinowski UH, Adler B (2013) The Viral Chemokine MCK-2 of Murine Cytomegalovirus Promotes Infection as Part of a gH/gL/MCK-2 Complex. PLoS Pathog 9:e1003493. doi: 10.1371/journal.ppat.1003493

Granstedt AE, Bosse JB, Thiberge SY, Enquist LW (2013) In vivo imaging of alphaherpesvirus infection reveals synchronized activity dependent on axonal sorting of viral proteins. Proc Natl Acad Sci USA 110:E3516–E3525.

Bosse JB, Ragues J, Wodrich H (2013) Fast Generation of Stable Cell Lines Expressing Fluorescent Marker Molecules to Study Pathogen Induced Processes. In: Bailer SM, Lieber D (eds) Virus-Host Interactions. Humana Press, Totowa, NJ, pp 153–169

2012

Marcinowski L, Lidschreiber M, Windhager L, Rieder M, Bosse JB, Rädle B, Bonfert T, Györy I, de Graaf M, Prazeres da Costa O, Rosenstiel P, Friedel CC, Zimmer R, Ruzsics Z, Dölken L (2012) Real-time transcriptional profiling of cellular and viral gene expression during lytic cytomegalovirus infection. PLoS Pathog 8:e1002908.

Pogoda M, Bosse JB, Wagner FM, Schauflinger M, Walther P, Koszinowski UH, Ruzsics Z (2012) Characterization of conserved region 2-deficient mutants of the cytomegalovirus egress protein pM53. J Virol 86:12512–12524.

Bosse JB, Bauerfeind R, Popilka L, Marcinowski L, Taeglich M, Jung C, Striebinger H, von Einem J, Gaul U, Walther P, Koszinowski UH, Ruzsics Z (2012) A beta-herpesvirus with fluorescent capsids to study transport in living cells. PLoS ONE 7:e40585.

2011

Maninger S, Bosse JB, Lemnitzer F, Pogoda M, Mohr CA, von Einem J, Walther P, Koszinowski UH, Ruzsics Z (2011) M94 is essential for the secondary envelopment of murine cytomegalovirus. J Virol 85:9254–9267.

2009

Lösing J-B, Di Fiore S, Ritter K, Fischer R, Kleines M (2009) Epstein–Barr virus BDLF2–BMRF2 complex affects cellular morphology. J Gen Virol 90:1440–1449.

2008

Gaspar M, Gill MB, Lösing J-B, May JS, and Stevenson PG (2008) Multiple functions for ORF75c in murid herpesvirus-4 infection. PLoS ONE 3:e2781.