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Filarsky Group

Systems Biology of Human Parasites

Prof. Dr. Michael Filarsky

Group Leader

+49 40 8998 87628
michael.filarsky@cssb-hamburg.de

Home Institute

Universität Hamburg
www.uni-hamburg.de/

We are employing a systematic approach to investigate, how the malaria parasite Plasmodium falciparum uses non-coding RNAs and its chromatin machinery to translate adaptive signals into changes of chromatin structure and gene expression. We are particularly interested into how the parasite uses these mechanisms to control cell cycle switching and the development of transmissible gametocyte stages.

Research Projects

PREVIOUS AND CURRENT RESEARCH

Malaria, caused by protozoan parasites of the genus Plasmodium continues to be a major global health burden, with an estimated 450,000 deaths annually and half of the world’s population at risk of infection. There is still no reliable vaccine available and resistance against frontline anti-malaria drugs is emerging. Thus, new drugs and intervention strategies are urgently needed, in order to fight the parasite and get closer to the goal of malaria eradication. The sexual gametocyte stages, which are taken up from the blood by Anopheles mosquitoes, are of special interest in this context. Being the only developmental stage of the parasite that is transmissible between human and mosquito hosts, they provide a prime target for new transmission-blocking intervention strategies. However, despite their high relevance for such strategies, the molecular mechanisms driving the switch from asexual replication to sexual development of malaria parasites remain poorly understood.

During asexual replication in human red blood cells, P. falciparum uses the conserved mechanism of heterochromatin protein 1 (HP1)-mediated gene silencing to epigenetically control sexual commitment and prevent the development of gametocytes. Changes in environmental conditions can lead to a remodelling of chromatin structure and the disassembly of heterochromatin across specific regions of the genome. This leads to a cell cycle switch and the activation of gametocyte development. Recent research from us and other laboratories identified key molecular factors involved in this process. In addition, we were able to show that regulatory long non-coding (lnc) RNAs play a part in this epigenetic network.

However, how environmental triggers set off this lncRNA-mediated regulation, how the involved factors are targeted to specific loci in the genome and what molecular machinery governs the establishment of active and inactive chromatin structures across the nucleus remains unknown. Our lab is focused on answering these questions, by investigating the formation of active and inactive chromatin structures and the molecular machinery governing these highly dynamic processes in P. falciparum. In addition, we are focused on uncovering the functions of lncRNAs in the regulation of these processes.

Ultimately, we believe that this will enable us to develop a broader understanding of the connection between environmental signals, changes of chromatin architecture and the epigenetic networks, which control sexual commitment and gametocyte development in malaria parasites.

KEY RESEARCH QUESTIONS

  • How is the expression of lncRNAs controlled?
  • What roles do lncRNAs play in the epigenetic control of gene expression and cell cycle control?
  • What is the molecular machinery governing chromatin dynamics in P. falciparum?

 

Research Team

3

Group Leader

Prof. Dr. Michael Filarsky
Phone:+49 40 8998 87628

E-Mail

Technical Staff

Monja Paasche
Phone:+49 40 8998 87629

E-Mail

PhD Student

Andres Lill
Phone:+49 40 8998 87629

E-Mail

Publications

2018

Filarsky M, Fraschka SA, Niederwieser I, Brancucci NMB, Carrington E, Carrió E, Moes S, Jenoe P, Bártfai R, Voss TS (2018) GDV1 induces sexual commitment of malaria parasites by antagonizing HP1-dependent gene silencing. Science 359:1259–1263. doi: 10.1126/science.aan6042

Fraschka SA, Filarsky M, Hoo R, Niederwieser I, Yam XY, Brancucci NMB, Mohring F, Mushunje AT, Huang X, Christensen PR, Nosten F, Bozdech Z, Russell B, Moon RW, Marti M, Preiser PR, Bártfai R, Voss TS (2018) Comparative Heterochromatin Profiling Reveals Conserved and Unique Epigenome Signatures Linked to Adaptation and Development of Malaria Parasites. Cell Host and Microbe 23:407–420.e8. doi: 10.1016/j.chom.2018.01.008

Maldonado R, Filarsky M, Grummt I, Längst G (2018) Purine– and pyrimidine–triple-helix-forming oligonucleotides recognize qualitatively different target sites at the ribosomal DNA locus. RNA 24:371–380. doi: 10.1016/S0162-0134(02)00444-0

2017

Ngwa CJ, Kiesow MJ, Papst O, Orchard LM, Filarsky M, Rosinski AN, Voss TS, Llinás M, Pradel G (2017) Transcriptional Profiling Defines Histone Acetylation as a Regulator of Gene Expression during Human-to-Mosquito Transmission of the Malaria Parasite Plasmodium falciparum. Front Cell Infect Microbiol 7:1600347. doi: 10.1002/med.214372

2015

Filarsky M, Zillner K, Araya I, Villar-Garea A, Merkl R, Längst G, Németh A (2015) The extended AT-hook is a novel RNA binding motif. RNA biology 12:864–876. doi: 10.1080/15476286.2015.1060394

2013

Zillner K, Filarsky M, Rachow K, Weinberger M, Längst G, Németh A (2013) Large-scale organization of ribosomal DNA chromatin is regulated by Tip5. Nucleic Acids Res 41:5251–5262. doi: 10.1093/nar/gkt218

2010

Weigert J, Neumeier M, Wanninger J, Filarsky M, Bauer S, Wiest R, Farkas S, Scherer MN, Schäffler A, Aslanidis C, Schölmerich J, Buechler C (2010) Systemic chemerin is related to inflammation rather than obesity in type 2 diabetes. Clin Endocrinol (Oxf) 72:342–348. doi: 10.1111/j.1365-2265.2009.03664.x

Weigert J, Obermeier F, Neumeier M, Wanninger J, Filarsky M, Bauer S, Aslanidis C, Rogler G, Ott C, Schäffler A, Schölmerich J, Buechler C (2010) Circulating levels of chemerin and adiponectin are higher in ulcerative colitis and chemerin is elevated in Crohn's disease. Inflamm Bowel Dis 16:630–637. doi: 10.1002/ibd.21091

2009

Huang KY, Filarsky M, Padula MP, Raftery MJ, Herbert BR, Wilkins MR (2009) Micropreparative fractionation of the complexome by blue native continuous elution electrophoresis. Proteomics 9:2494–2502. doi: 10.1002/pmic.200800525

Weigert J, Neumeier M, Wanninger J, Schober F, Sporrer D, Weber M, Schramm A, Wurm S, Stögbauer F, Filarsky M, Schäffler A, Aslanidis C, Schölmerich J, Buechler C (2009) Adiponectin upregulates monocytic activin A but systemic levels are not altered in obesity or type 2 diabetes. Cytokine 45:86–91. doi: 10.1016/j.cyto.2008.10.017

2008

Weigert J, Neumeier M, Wanninger J, Wurm S, Kopp A, Schober F, Filarsky M, Schäffler A, Zeitoun M, Aslanidis C, Buechler C (2008) Reduced response to adiponectin and lower abundance of adiponectin receptor proteins in type 2 diabetic monocytes. FEBS Lett 582:1777–1782. doi: 10.1016/j.febslet.2008.04.031